Author (s): M. Z. Hossain1, K. M. Nasiruddin2, M.S. Haque2, A. Khatun3
Abstract
The research was conducted during December 2008 to November 2009 at Biotechnology Department, Bangladesh Agricultural University (BAU), Mymensingh. Corchorus capsularis (C83, BJC-7370) were used to observe their transformation ability. Cotyledons (with attached petiole) were used as explants. An efficient and reproducible protocol for the production of transgenic jute plant was developed by inoculating petiole with cotyledon into Agrobacterium tumefaciens strain LBA4404 carrying a binary vector pBI121, which contains selectable marker gene nptII conferring resistance to kanamycin and the GUS reporter gene. After co-cultivation and selection, histochemical GUS assay was performed in two varieties (viz. C-83, BJC-7370). In the transformed explants, GUS reporter gene was expressed showing blue color in the explant tissues. Non-transformed explants did not show any color. Among the varieties, C-83 showed thehighest response to GUS assay (66.33%).
Key words: Cotyledons, Transgenic plant, Vector, Explant
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J. Expt. Biosci.1(2):67-72, July 2010
