ROLE
OF DEHYDRATION RESPONSIVE ELEMENT BINDING PROTEIN-1 IN TOMATO TREATED WITH
VARIOUS ABIOTIC STRESSES
M. S. Islam* and M. H. Wang
*Corresponding
author: E-mail: saiful_sau@yahoo.com
Abstract
DREB1
(dehydration responsive element binding protein-1) plays important roles in the regulation of plant resistance to various
abiotic stresses. In the present investigation, a cDNA clone coding a LeDREB1 gene was isolated from a cDNA
library of tomato leaves (Lycopersicon
esculentum) and named as LeDREB1 and its sequence signatures and expression
patterns of the transcripts were observed under various abiotic stresses. This
gene contains an open reading frame (ORF) of 301 amino acids encoding a DREB1
protein. LeDREB1 contained one conserved AP2/ERF domain and
its amino acid sequence was similar to the DREB1 group of the DREB family from Medicago
truncatula, oryza sativa,
Hordeum vulgare
subsp. vulgare, glycine max, and the cynodon
dactylon. Genomic
DNA-blot analysis for LeDREB1 suggested
one locus in the Lycopersicon
esculentum genome. The tissue specific expression profiling indicated that LeDREB1 was expressed
in
different parts and especially the
highest expression was found in young leaves and roots and weak expression in
stem and mature leaves. Transcription of LeDREB1 was induced in response to high salinity, drought, cold,
but not ABA. These results demonstrate that the LeDREB-1 gene may be responses to abiotic stresses in tomato and useful in improving of plant tolerance
to abiotic stresses.
Key words: DREB1, Tomato, Abiotic stresses and Expression
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