S. H. Anik, M. S.
Ahmed, F. Khatun, H. M. M. Tariq Hossain* and M. E. Hoque
*Corresponding
author’s Email: hmmtariq@yahoo.com
Abstract
The study was
undertaken to evaluate the effect of Benzyl adenine (BA)
Indole-3-Butyric acid (IBA) on in vitro plantlet regeneration of
Turmeric. The healthy, disease free sprouts of turmeric were used as
explants which were inoculated in Murashige and Skoog (MS) media
supplemented with different concentration of BA (1.0, 2.0, 3.0 and
4.0 mg/L) and IBA (1.0, 2.0, 3.0 and 4.0 mg/L). The experiments were
arranged in Completely Randomized Design (CRD) with three
replications. The maximum shoot induction was observed with 2.0mg/L
BA. The highest shoot length (5.93 cm) was found in BA 2.0 mg/L + IBA
3.0mg/L and that of the minimum (1.4 cm) was detected in control
medium. The shoot lengths (3.4cm, 5cm and 5.9cm) at 21, 28 and 42
days after induction (DAI) respectively were found in BA 2.0 mg/L +
IBA 3.0mg/L. The medium prepared with BA 2.0 mg/L + IBA 1.0 mg/L
showed the highest number of root (6.0, 8.6, 12.67) at 21, 28 and 42
DAI respectively. The survival rates of the regenerated plantlets in
growth chamber, in shade house and in field conditions were 100%,
86.66% and 83% respectively. Therefore, the protocol developed for in
vitro propagation appears with high commercial value for year round
production of Turmeric in Bangladesh.
Keywords: In virto
regeneration, Turmeric
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