Author (s): L. Yeasmin1, S. Ahmed2, M.H. Rashid3, S. Parveen4 and N. Zeba3*
*Corresponding author, E-mail: naheed0359@hotmail.com
*Corresponding author, E-mail: naheed0359@hotmail.com
Abstract
Microtuber induction and its growth and development in different nitrogen and potassium levels were studied at the Bangladesh Agricultural Research Institute during 2005. Three levels each of nitrogen (30, 60 and 90 mM N/l) and potassium (10, 20 and 30 mM K/l) with nine treatment combinations were used. MS media supplemented with 500mg/l CCC, 5 mg/l BAP and 8% sucrose were used for culture. The cultures were incubated in complete dark at a temperature of 20±1 oC. But the microplants were incubated in 3000 lux light intensity for 16 h photoperiod with a constant temperature of 22 ± 2 oC. Initiation of microtuber occurred most early with N60 x K20 (11.50-13.62 days) compared to N30 or N90 with all levels of K (approx.15-19 days). N60 with all levels of K produced maximum number of tuber per tube (2.25) with tuber weight 145.75 mg and mean tuber weight 71.08 mg. All the N x K levels gave comparatively larger microtuber after 8 weeks of culture than 6 weeks. N30 and N90 with all levels of K producing high proportion of <50 mg size microtuber (9.45-100%) while N60 with all levels of K produced high proportion of 100-150 mg size microtuber (max. 60.82%).
J. Expt. Biosci. 2(1): January 2011
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