INFECTIVITY OF AGROBACTERIAL
STRAINS TO HYPOCOTYL EXPLANTS
FOR TRANSGENIC LEAFY PERILLA
H.M.M. Tariq Hossain,
Y. H. Kim and Y. S. Lee*
*Corresponding author, Email:
mariolee@sch.ac.kr
Abstract
Agrobacterium
mediated gene delivery has been a widely popular tool in plant molecular
biology to develop genetically modified crops globally. In this study we
compared the efficiencies of two Agrobacterial strains of Agropine – type (EHA
105) and Nopaline – type (GV 3101) while transforming the Korean leafy perilla
plants with pCAMBIA3300 inserted with homogentisic acid geranylgeranyl transferase
(HGGT) gene cassette. The efficacies of the strains were compared by their relative
infectivity of hypocotyl explants selected with Phosphinothricin (PPT) in the
MS media as a selectable marker. However, the percentages of explants inducing
shoots were varied in number and were found to be the Agrobacterial
strain-dependent. The highest mean numbers (55.06%) of survived explants were
scored with GV3101, which was significantly higher than that of EHA 105 (37.07).
Thus, the assessment of Agrobacterial infectivity in terms of higher mortality
rate (62.93%) of explants co-cultivated with agropine-type strain EHA 105
revealed that the nopaline–type strain GV3101 was more efficient in
transforming the leafy Perilla. Transgenically engineered plants were recovered
from the explants in vitro infected
by the GV3101 strain of Agrobacterium only.
Key
words: Agrobacterial strains, infectivity,
hypocotyl explants and Perilla.
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